Renato Dulbecco and the Impact of Dulbecco’s Modified Eagle’s Medium (DMEM) on Cell Culture

14 January 25 Useful information ، Terminology ، Tools and Materials ، Renato Dulbecco CellCulture ، کشت_سلولی ، جایزه_نوبل ، تحقیقات_سرطان ، DMEM ، رناتو_دولبکو ، ویروس‌شناسی ، RenatoDulbecco ، Virology ، CancerResearch ، NobelPrize
Renato Dulbecco and the Impact of Dulbecco’s Modified Eagle’s Medium (DMEM) on Cell Culture
Renato Dulbecco:   Renato Dulbecco, an Italian-American virologist, was born on February 22, 1914, in Catanzaro, Italy. He passed away on February 19, 2012, in La Jolla, California. In 1975, Dulbecco was awarded the Nobel Prize in Physiology or Medicine for his groundbreaking work on oncoviruses—viruses capable of inducing cancer. His pioneering research has contributed significantly to the fields of cell biology and virology.   One of his key contributions …
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Growth Media and Differentiation Media in Neuromuscular Labs

14 January 25 Useful information ، Terminology ، Tools and Materials ، Growth Media and Differentiation Media ، Renato Dulbecco skeletalmuscle ، SkeletalMuscle ، CellCulture ، کشت_سلولی ، ScientificTools ، پژوهش_عصبی_عضلانی ، محیط_رشد ، ابزارهای_علمی ، محیط_افتراقی ، GrowthMedia ، NeuromuscularResearch ، DifferentiationMedia
Growth Media and Differentiation Media in Neuromuscular Labs
  In neuromuscular research, selecting the appropriate culture media is essential for optimal cell maintenance and functional studies. Two key types of media commonly used are "growth media" and "differentiation media", each serving distinct purposes.   "Growth Media:" Growth media is designed to support the proliferation and maintenance of skeletal muscle cells. It typically consists of:   - Dulbecco’s Modified Eagle’s Medium/Nutrient Mixture F-12 (DMEM/F-12) with 2.5 mM L-glutamine and …
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Immortalized Skeletal Muscle Cells: Advancing DMD Research 

13 January 25 Useful information ، Terminology ، Tools and Materials ، Immortalized Skeletal Muscle Cells DuchenneMuscularDystrophy ، ExonSkipping ، musculardystrophy ، دیستروفی_عضلانی_دوشن ، DMDResearch ، MuscularDystrophy ، حذف_اگزون ، NeuromuscularScience ، CellModels ، ScientificTools ، پژوهش_عصبی_عضلانی ، سلولهای_جاودانه ، پژوهش_دوشن
Immortalized Skeletal Muscle Cells: Advancing DMD Research 
    Immortalized Skeletal Muscle Cells: Advancing DMD Research    Immortalized skeletal muscle cells derived from both healthy individuals and Duchenne muscular dystrophy (DMD) patients offer a powerful platform for scientific discovery in the field of neuromuscular disorders. These cells provide a reproducible model for studying disease mechanisms and investigating therapeutic strategies, particularly in the context of exon-skipping therapies.     By using these immortalized cells, researchers can explore how …
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Cell Scrapers

08 January 25 Useful information ، Terminology ، Tools and Materials ، Cell Scrapers BiomedicalResearch ، ResearchTools ، CellCulture ، LaboratoryTech ، CellScrapers ، CellHarvesting ، NonEnzymaticMethods ، MolecularBiology ، کشت_سلولی ، اسکرپرهای_سلولی ، روش‌های_غیر_آنزیمی ، تحقیقات_پزشکی
Cell Scrapers
  Get Acquainted with Essential Scientific Tools and Materials: Cell Scrapers in Research Labs   Cell scrapers are indispensable tools in laboratory cell culture, primarily used for harvesting adherent cells from the surface of culture vessels such as petri dishes, flasks, or multi-well plates. Made from non-toxic, medical-grade plastic, these scrapers feature a flat, rigid blade that allows researchers to gently dislodge cells without damaging them, offering a non-enzymatic alternative …
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Anti-dystrophin Antibodies and Their Applications in Western Blotting for Quantifying Dystrophin Rescue

06 January 25 Useful information ، Terminology ، Tools and Materials ، Anti-dystrophin Antibodies DuchenneMuscularDystrophy ، GeneTherapy ، ExonSkipping ، دیستروفی_عضلانی_دوشن ، DMDResearch ، Dystrophin ، ژن‌درمانی ، حذف_اگزون ، دیستروفین ، WesternBlotting ، Antibodies ، ProteinQuantification ، وسترن_بلات ، آنتی‌بادی‌ها
Anti-dystrophin Antibodies and Their Applications in Western Blotting for Quantifying Dystrophin Rescue
Dystrophin, a crucial protein for maintaining muscle integrity, is severely deficient in Duchenne Muscular Dystrophy (DMD) patients. Quantifying dystrophin restoration following therapeutic interventions is essential for evaluating treatment efficacy. Western blotting, a widely used protein analysis technique, plays a pivotal role in this quantification, with anti-dystrophin antibodies being central to its success.   Anti-dystrophin antibodies are specifically designed to detect and bind dystrophin protein, making them indispensable for Western blot …
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Systemic delivery of an AAV9 exonskipping vector significantly improves or prevents features of Duchenne muscular dystrophy in the Dup2 mouse

05 January 25 Articles ، Kevin Flanigan ، Robert Weiss DMD ، GeneTherapy ، ExonSkipping ، دیستروفی_عضلانی_دوشن ، حذف_اگزون ، ژن_درمانی ، DystrophinRestoration ، بازسازی_دیستروفین ، Dup2MouseModel ، AAV9 ، U7snRNA ، مدل_موش_Dup2
Systemic delivery of an AAV9 exonskipping vector significantly improves or prevents features of Duchenne muscular dystrophy in the Dup2 mouse
  Background: Duchenne muscular dystrophy (DMD) is a severe genetic disorder caused by mutations in the DMD gene. These mutations disrupt the dystrophin protein, essential for muscle function. Current treatments, like exon-skipping oligonucleotides, have limitations due to their short lifespan and need for frequent administration.   Objective: This study explored the use of a self-complementary adeno-associated virus (scAAV9) vector expressing U7 small nuclear RNA (snRNA) to target exon 2 of …
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Increase in Full-Length Dystrophin by Exon Skipping in Duchenne Muscular Dystrophy Patients with Single Exon Duplications: An Open-label Study

02 January 25 Articles ، Linda Pax Lowes ، Kevin Flanigan DuchenneMuscularDystrophy ، GeneTherapy ، ExonSkipping ، AntisenseOligonucleotides ، دیستروفی_عضلانی_دوشن ، درمان_ژنی ، الیگونوکلئوتید_آنتی_سنس ، Golodirsen ، Casimersen ، پرش_اگزون ، Dystrophin ، ClinicalTrial ، کازیمیرسن ، گولودیرسن
Increase in Full-Length Dystrophin by Exon Skipping in Duchenne Muscular Dystrophy Patients with Single Exon Duplications: An Open-label Study
1. Introduction Duchenne Muscular Dystrophy (DMD) is a severe genetic disorder predominantly affecting boys, with an incidence of approximately 1 in 5,000 male births. It is caused by mutations in the DMD gene that lead to disrupted production of dystrophin, a protein essential for muscle stability. Exon skipping is a therapeutic strategy that restores the reading frame of the mutated gene to enable dystrophin production. This study focuses on applying …
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Challenges of Assessing Exon 53 Skipping of the HumanDMD Transcript with Locked Nucleic Acid-ModifiedAntisense Oligonucleotides in a Mouse Modelfor Duchenne Muscular Dystrophy

02 January 25 Articles ، Annemieke Aartsma-Rus ، Anastasia Khvorova ، Masad J. Damha DMD ، ExonSkipping ، AntisenseTherapy ، حذف_اگزون ، DystrophinRestoration ، بازسازی_دیستروفین ، LNA ، PreclinicalStudy ، دیستروفی_دوشن ، الیگونوکلئوتید
Challenges of Assessing Exon 53 Skipping of the HumanDMD Transcript with Locked Nucleic Acid-ModifiedAntisense Oligonucleotides in a Mouse Modelfor Duchenne Muscular Dystrophy
  Duchenne muscular dystrophy (DMD) is a severe genetic disorder caused by mutations in the dystrophin gene, leading to muscle degeneration and early death. Antisense oligonucleotides (AONs) are promising therapeutic agents designed to skip faulty exons in the dystrophin gene and restore functional protein.   This study evaluated the efficiency of AONs targeting exon 53 of the dystrophin gene in a mouse model, using chemically modified AONs to enhance skipping …
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Rare Diseases and Orphan Diseases

28 December 24 Useful information ، muscle diseases ، Terminology Rare Diseases ، Orphan Diseases ، بیماری‌های نادر ، بیماری‌های یتیم
Rare Diseases and Orphan Diseases
  "Rare Diseases" and "Orphan Diseases" are terms often used interchangeably, but they have distinct meanings and implications, particularly in the context of medical research and drug development. Here's an explanation of both, along with their differences:   Rare Diseases - Definition: A rare disease is one that affects a small percentage of the population. The exact threshold for rarity varies by region:   - United States: Affects fewer than …
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Next Generation Exon 51 Skipping Antisense Oligonucleotides for Duchenne Muscular Dystrophy

28 December 24 Articles ، Annemieke Aartsma-Rus ، Judith van Deutekom DuchenneMuscularDystrophy ، ExonSkipping ، دیستروفی_عضلانی_دوشن ، antisenseoligonucleotide ، پرش_اگزون ، AntisenseOligonucleotide ، DystrophinRestoration ، NextGenerationTherapies ، PreclinicalResearch ، الیگونوکلئوتیدآنتی_سنس ، بازگردانی_دیستروفین ، تحقیقات_پیش_بالینی
Next Generation Exon 51 Skipping Antisense Oligonucleotides for Duchenne Muscular Dystrophy
Antisense oligonucleotides (AONs) have emerged as a promising therapy for Duchenne Muscular Dystrophy (DMD), a severe neuromuscular disease caused by dystrophin deficiency. Early AON drugs like drisapersen and eteplirsen achieved limited success, leading researchers to develop more efficient next-generation AONs. This study focused on refining AONs for exon 51 skipping through advanced chemical modifications and identifying optimal target sites to enhance efficacy and safety.   Researchers screened over 100 AONs …
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